Potential neuron-autonomous Purkinje cell degeneration by 2′,3′-cyclic nucleotide 3′-phosphodiesterase promoter/Cre-mediated autophagy impairments

Title
Potential neuron-autonomous Purkinje cell degeneration by 2′,3′-cyclic nucleotide 3′-phosphodiesterase promoter/Cre-mediated autophagy impairments
Authors
이경은오유나조영래Juyeon JoHye Ran KimSo Young JangHana GoMin-Young SongDa Kyeong ParkYoon Kyung ShinSung Joong LeeSang-Myung CheonHyun Kyoung LeeYoung Hye KimHwan Tae Park
Issue Date
2021-01
Publisher
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Citation
VOL 35, NO 1, e21225
Abstract
Studies of neuroglial interaction largely depend on cell-specific gene knockout (KO) experiments using Cre recombinase. However, genes known as glial-specific genes have recently been reported to be expressed in neuroglial stem cells, leading to the possibility that a glia-specific Cre driver results in unwanted gene deletion in neurons, which may affect sound interpretation. 2′,3′-Cyclic nucleotide 3′-phosphodiesterase (CNP) is generally considered to be an oligodendrocyte (OL) marker. Accordingly, Cnp promoter-controlled Cre recombinase has been used to create OL-specific gene targeting mice. However, in this study, using Rosa26-tdTomato-reporter/Cnp-Cre mice, we found that many forebrain neurons and cerebellar Purkinje neurons belong to the lineages of Cnp-expressing neuroglial stem cells. To answer whether gene targeting by Cnp-Cre can induce neuron-autonomous defects, we conditionally deleted an essential autophagy gene, Atg7, in Cnp-Cre mice. The Cnp-Cre-mediated Atg7 KO mice showed extensive p62 inclusion in neurons, including cerebellar Purkinje neurons with extensive neurodegeneration. Furthermore, neuronal areas showing p62 inclusion in Cnp-Cre-mediated Atg7 KO mice overlapped with the neuronal lineage of Cnp-expressing neuroglial stem cells. Moreover, Cnp-Cre-mediated Atg7-KO mice did not develop critical defects in myelination. Our results demonstrate that a large population of central neurons are derived from Cnp-expressing neuroglial stem cells; thus, conditional gene targeting using the Cnp promoter, which is known to be OL-specific, can induce neuron-autonomous phenotypes.
URI
http://pubs.kist.re.kr/handle/201004/74221
ISSN
0892-6638
Appears in Collections:
KIST Publication > Article
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