Park, In Su Kim, Soo Hyun Kim, Sang-Heon Han, Jeong Woo Chung Eunna KO YOUNG-GUN 2024-01-13T05:31:18Z 2024-01-13T05:31:18Z 2021-09-29 https://pubs.kist.re.kr/handle/201004/103043 Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 ？m, 100-200 ？m, and 300-500 ？m, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method. English hydrogel collagen smooth muscle cells vascular tissue engineering pore size Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering Conference 2 한국조직공학ㆍ재생의학회 한국조직공학ㆍ재생의학회 KO