Cho, Kyungjin Cho, Minkee Kaiyrlinova, Shugyla Park, Jihye Park, Suin Park, Nohback Bae, Hyokwan 2024-01-19T08:03:08Z 2024-01-19T08:03:08Z 2023-11-30 2023-12 0960-8524 https://pubs.kist.re.kr/handle/201004/113043 The aim of this study was to propose repeated and prolonged batch (RPB) assay as a promising specific anammox activity (SAA) methodology assessing the anammox activity under stressed salinity and pH conditions. Response surface analysis (RSA) was used as a regression tool to evaluate statistical significance. The feasibility of RPB was investigated at 0 to 15 g-NaCl/L of salinity and pH 6 to 8 with reflecting the results of preliminary SAA. As a result, conventional SAA was statistically insignificant. In addition, the RSA results obtained from repeated batch did not meet the statistical significance despite ten times iterative reaction. Interestingly, the RPB assay (i.e., applied both repeated and prolonged reaction) was effective to obtain the reliable results. Candidadus Brocadia and Candidadus Jettenia were functional anammox microbiome during RPB. Outcomes of this study suggest that RPB assay can be applied to accurately determine the anammox activity under various stressful conditions. English Elsevier BV Improved regression model for anaerobic ammonium oxidation by repeated and prolonged batch assay under stressful salinity and pH conditions Article 10.1016/j.biortech.2023.129896 1 Bioresource Technology, v.390 Bioresource Technology 390 N scie scopus 001102807300001 2-s2.0-85175163672 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article NITROGEN REMOVAL ANAMMOX PROCESS PERFORMANCE INHIBITION BACTERIA Specific anammox activity Response surface analysis Stressed environmental conditions Statistical significance Anammox microbiome