Kang, Minchul Nam, Donghyeon Ahn, Jeongyeon Chung, Yoon Jang Lee, Seung Woo Choi, Young-Bong Kwon, Cheong Hoon Cho, Jinhan 2024-01-19T08:03:28Z 2024-01-19T08:03:28Z 2023-11-17 2023-12 0935-9648 https://pubs.kist.re.kr/handle/201004/113052 Biofuel cells (BFCs) based on enzymatic electrodes hold great promise as power sources for biomedical devices. However, their practical use is hindered by low electron transfer efficiency and poor operational stability of enzymatic electrodes. Here, a novel mediator-free multi-ply BFC that overcomes these limitations and exhibits both substantially high-power output and long-term operational stability is presented. The approach involves the utilization of interfacial interaction-induced assembly between hydrophilic glucose oxidase (GOx) and hydrophobic conductive indium tin oxide nanoparticles (ITO NPs) with distinctive shapes, along with a multi-ply electrode system. For the preparation of the anode, GOx and oleylamine-stabilized ITO NPs with bipod/tripod type are covalently assembled onto the host fiber electrode composed of multi-walled carbon nanotubes and gold (Au) NPs. Remarkably, despite the contrasting hydrophilic and hydrophobic properties, this interfacial assembly approach allows for the formation of nanoblended GOx/ITO NP film, enabling efficient electron transfer within the anode. Additionally, the cathode is prepared by sputtering Pt onto the host electrode. Furthermore, the multi-ply fiber electrode system exhibits unprecedented high-power output (approximate to 10.4 mW cm-2) and excellent operational stability (2.1 mW cm-2, approximate to 49% after 60 days of continuous operation). The approach can provide a basis for the development of high-performance BFCs. In this study, a groundbreaking mediator-free hybrid biofuel cell that demonstrates exceptional performance is introduced. Through a multi-ply electrode design and an innovative assembly approach involving hydrophobic conductive oxide nanoparticles with distinct shapes and hydrophilic glucose oxidases, significant enhancements in power output and operation stability are achieved for cotton fiber-based biofuel cells.image English WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim A Mediator-Free Multi-Ply Biofuel Cell Using an Interfacial Assembly between Hydrophilic Enzymes and Hydrophobic Conductive Oxide Nanoparticles with Pointed Apexes Article 10.1002/adma.202304986 1 Advanced Materials, v.35, no.51 Advanced Materials 35 51 Y scie scopus 001087283100001 2-s2.0-85174831177 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article GLUCOSE-OXIDASE REDOX CENTERS ELECTRON-TRANSFER CARBON FILMS FABRICATION BIOANODE a mediator-free biofuel cell a multi-ply electrode conductive oxide nanoparticles enzymes