Yang, Da Som Wu, Yixin Kanatzidis, Evangelos E. Avila, Raudel Zhou, Mingyu Bai, Yun Chen, Shulin Sekine, Yurina Kim, Joohee Deng, Yujun Guo, Hexia Zhang, Yi Ghaffari, Roozbeh Huang, Yonggang Rogers, John A. 2024-01-19T08:30:34Z 2024-01-19T08:30:34Z 2023-10-29 2023-11 2051-6347 https://pubs.kist.re.kr/handle/201004/113135 Systems for capture, storage and analysis of eccrine sweat can provide insights into physiological health status, quantify losses of water, electrolytes, amino acids and/or other essential species, and identify exposures to adverse environmental species or illicit drugs. Recent advances in materials and device designs serve as the basis for skin-compatible classes of microfluidic platforms and in situ colorimetric assays for precise assessments of sweat rate, sweat loss and concentrations of wide-ranging types of biomarkers in sweat. This paper presents a set of findings that enhances the performance of these systems through the use of microfluidic networks, integrated valves and microscale optical cuvettes formed by three dimensional printing in hard/soft hybrid materials systems, for accurate spectroscopic and fluorometric assays. Field studies demonstrate the capability of these microcuvette systems to evaluate the concentrations of copper, chloride, and glucose in sweat, along with the pH of sweat, with laboratory-grade accuracy and sensitivity. 3D-printed microfluidic systems that incorporate rigid polymeric structures in soft elastomeric encapsulation layers gently bond to the skin, to capture sweat and provide the basis for accurate, spectroscopic measurements of sweat biomarkers. English Royal Society of Chemistry 3D-printed epidermal sweat microfluidic systems with integrated microcuvettes for precise spectroscopic and fluorometric biochemical assays Article 10.1039/D3MH00876B 1 Materials Horizons, v.10, no.11, pp.4992 - 5003 Materials Horizons 10 11 4992 5003 N scie scopus 001077237600001 Chemistry, Multidisciplinary Materials Science, Multidisciplinary Chemistry Materials Science Article; Early Access ENZYME IMMOBILIZATION SKIN ABSORPTION SURFACE COPPER SOFT