Kim, Moon-Ju Bae, Hyung Eun Kwon, Soonil Park, Mi-Kyung Yong, Dongeun Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T08:32:04Z 2024-01-19T08:32:04Z 2023-10-05 2023-10 0956-5663 https://pubs.kist.re.kr/handle/201004/113208 The use of phages-a natural predator of bacteria-has emerged as a therapeutic strategy for treating multidrugresistant bacterial infections; thus, the isolation and detection of phages from the environment is crucial for advancing phage therapy. Herein, for the first time, we propose a nanoplasmonic-based biodetection platform for phages that utilizes bacterial outer membranes (OMs) as a biorecognition element. Conventional biosensors based on phage-bacteria interactions encounter multiple challenges due to the bacteriolytic phages and potentially toxic bacteria, resulting in instability and risk in the measurement. Therefore, instead of whole living bacteria, we employ a safe biochemical OMs fraction presenting phage-specific receptors, allowing the robust and reliable phage detection. In addition, the biochip is constructed on bimetallic nanoplasmonic islands through solid-state dewetting for synergy between Au and Ag, whereby sensitive detection of phage-OMs interactions is achieved by monitoring the absorption peak shift. For high detection performance, the nanoplasmonic chip is optimized by systematically investigating the morphological features, e.g., size and packing density of the nanoislands. Using our optimized device, phages are detected with high sensitivity (& GE;-104 plaques), specificity (little cross-reactivity), and affinity (stronger binding to the host OMs than anti-bacterial antibodies), further exhibiting the cell-killing activities. English Pergamon Press Ltd. Phage-targeting bimetallic nanoplasmonic biochip functionalized with bacterial outer membranes as a biorecognition element Article 10.1016/j.bios.2023.115598 1 Biosensors and Bioelectronics, v.238 Biosensors and Bioelectronics 238 N scie scopus 001063371400001 2-s2.0-85167968598 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article SURFACE-PLASMON RESONANCE NANOPARTICLES RESISTANCE AU ABLATION THERAPY INNATE OPTICS Bacterial outer membranes Localized surface plasmon resonance Bimetallic nanoplasmonic islands Phage-targeting biosensor Anti-Bacterial antibody