Rheem, Hyeong Bin Choi, Hyunwoo Yang, Seoin Han, Sol Rhee, Su Yeon Jeong, Hyeongseop Lee, Kyung-Bok Lee, Yeji Kim, In-San Lee, Hojae Choi, Insung S. S. 2024-01-19T08:32:52Z 2024-01-19T08:32:52Z 2023-06-22 2023-10 1613-6810 https://pubs.kist.re.kr/handle/201004/113248 Manipulation and control of cell chemotaxis remain an underexplored territory despite vast potential in various fields, such as cytotherapeutics, sensors, and even cell robots. Herein is achieved the chemical control over chemotactic movement and direction of Jurkat T cells, as a representative model, by the construction of cell-in-catalytic-coat structures in single-cell nanoencapsulation. Armed with the catalytic power of glucose oxidase (GOx) in the artificial coat, the nanobiohybrid cytostructures, denoted as Jurkat([Lipo_GOx]), exhibit controllable, redirected chemotactic movement in response to d-glucose gradients, in the opposite direction to the positive-chemotaxis direction of naive, uncoated Jurkat cells in the same gradients. The chemically endowed, reaction-based fugetaxis of Jurkat([Lipo_GOx]) operates orthogonally and complementarily to the endogenous, binding/recognition-based chemotaxis that remains intact after the formation of a GOx coat. For instance, the chemotactic velocity of Jurkat([Lipo_GOx]) can be adjusted by varying the combination of d-glucose and natural chemokines (CXCL12 and CCL19) in the gradient. This work offers an innovative chemical tool for bioaugmenting living cells at the single-cell level through the use of catalytic cell-in-coat structures. English Wiley - V C H Verlag GmbbH & Co. Fugetaxis of Cell-in-Catalytic-Coat Nanobiohybrids in Glucose Gradients Article 10.1002/smll.202301431 1 Small, v.19, no.41 Small 19 41 N scie scopus 001002131500001 2-s2.0-85161054412 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article VASCULAR SMOOTH-MUSCLE SIGNALING PATHWAYS CHEMOTAXIS MIGRATION RECEPTOR CXCL12 ACTIVATION MOVEMENT MOTILITY CCL19 catalysis chemokines chemotaxis liposomes single-cell nanoencapsulation