Jun, Soyoung Kim, Muwoong Park, Heeyoun Hwang, Eunmi Yamamoto, Yukio Tanaka-Yamamoto, Keiko 2024-01-19T08:33:20Z 2024-01-19T08:33:20Z 2023-10-29 2023-09 2211-1247 https://pubs.kist.re.kr/handle/201004/113269 As cerebellar granule cells (GCs) coordinate the formation of regular cerebellar networks during postnatal development, molecules in GCs are expected to be involved. Here, we test the effects of the knockdown (KD) of multiple epidermal growth factor-like domains protein 11 (MEGF11), which is a homolog of proteins mediating astrocytic phagocytosis but is substantially increased at the later developmental stages of GCs on cerebellar development. MEGF11-KD in GCs of developing mice results in abnormal cerebellar structures, including extensively ectopic Purkinje cell (PC) somas, and in impaired motor functions. MEGF11-KD also causes abnormally asynchronous synaptic release from GC axons, parallel fibers, before the appearance of abnormal cerebellar structures. Interestingly, blockade of this abnormal synaptic release restores most of the cerebellar structures. Thus, apart from phagocytic functions of its related homologs in astrocytes, MEGF11 in GCs promotes proper PC development and cerebellar network formation by regulating immature synaptic transmission. English Cell Press Organization of Purkinje cell development by neuronal MEGF11 in cerebellar granule cells Article 10.1016/j.celrep.2023.113137 1 Cell Reports, v.42, no.9 Cell Reports 42 9 Y scie scopus 001078899200001 2-s2.0-85170696411 Cell Biology Cell Biology Article GENE-EXPRESSION POSTNATAL-DEVELOPMENT SYNAPSE ELIMINATION SPATIAL-ORGANIZATION MICE LACKING RECEPTOR PARALLEL RELEASE CORTEX GLUR-DELTA-2