Lee, Sang Heon Oh, Youngse Bong, Sim-Kyu Lee, Jin Woo Park, No-June Kim, Young-Joo Park, Hyun Bong Kim, Yong Kee Kim, Seung Hyun Kim, Su-Nam 2024-01-19T08:33:30Z 2024-01-19T08:33:30Z 2023-10-29 2023-09 2468-0834 https://pubs.kist.re.kr/handle/201004/113278 Various allergic diseases such as atopic dermatitis (AD), allergic rhinitis, and asthma are considered incurable conditions that have yet to be fully conquered. Paedoksan (PDS), an herbal preparation consisting of 14 medicines, displays effective anti-inflammatory and anti-allergic properties, yet its underlying molecular mechanism is unknown. This study aims to uncover PDS's mechanism for treating allergic diseases and suggest its therapeutic potential. Through a network pharmacological prediction, its impact on signal transducer and activator of transcription 6 (STAT6) regulation, a sub-mechanism of interleukin 4 (IL-4), a major inflammatory cytokine involved in degranulation and allergy, was investigated in RBL-2H3 cells and an atopic mouse model. PDS inhibits immunoglobulin E (IgE)-induced degranulation and STAT6 phosphorylation evoked by IL-4 in granulocytes. The downregulation of phospho-STAT6 and thymic stromal lymphopoietin (TSLP) by PDS was confirmed in 2,4-dinitrochlorobenzene (DNCB)-induced mouse skin. The results demonstrate that PDS exhibited remarkable effects on degranulation and STAT6 phosphorylation in RBL-2H3 cells, as well as in an atopic mouse model. Furthermore, the main active components from PDS based on chromatographic analysis showed good accordance with PDS's effects on RBL-2H3 cells. In summary, these findings collectively suggest that PDS holds the potential to effectively suppress inflammatory and allergic reactions by obstructing the target IL-4 protein and its downstream effects, as elucidated through a network pharmacological analysis. English 한국응용생명화학회 Paedoksan ameliorates allergic disease through inhibition of the phosphorylation of STAT6 in DNCB-induced atopic dermatitis like mice Article 10.1186/s13765-023-00815-0 1 Applied Biological Chemistry, v.66, no.1 Applied Biological Chemistry 66 1 Y scie scopus kci ART002997100 001067589900002 2-s2.0-85171369234 Food Science & Technology Food Science & Technology Article MAST-CELLS INFLAMMATION MECHANISMS MEDICINE IGE Paedoksan Network pharmacology Atopic dermatitis STAT6 IL-4