Choi, Yunyoung Song, Younseong Cho, Younghak Choi, Kyung-Hak Park, Chulmin Lee, Dong-Gun Lee, Raeseok Choi, Nakwon Kang, Ji Yoon Im, Sung Gap Seong, Hyejeong 2024-01-19T08:33:39Z 2024-01-19T08:33:39Z 2023-10-27 2023-09 0003-2700 https://pubs.kist.re.kr/handle/201004/113287 The ongoing coronavirus disease 2019 (COVID-19) pandemic demands rapid and straightforward diagnostic tools to prevent early-stage viral transmission. Although nasopharyngeal swabs are a widely used patient sample collection method for diagnosing COVID-19, using these samples for diagnosis without RNA extraction increases the risk of obtaining false-positive and -negative results. Thus, multiple purification steps are necessary, which are time-consuming, generate significant waste, and result in substantial sample loss. To address these issues, we developed surface-modified polymerase chain reaction (PCR) tubes using the tertiary aminated polymer poly-(2-dimethylaminomethylstyrene) (pDMAMS) via initiated chemical vapor deposition. Introducing the clinical samples into the pDMAMS-coated tubes resulted in approximately 100% RNA capture efficiency within 25 min, which occurred through electrostatic interactions between the positively charged pDMAMS surface and the negatively charged RNA. The captured RNA is then detected via chamber digital PCR, enabling a sensitive, accurate, and rapid diagnosis. Our platform provides a simple and efficient RNA extraction and detection strategy that allows detection from 22 nasopharyngeal swabs and 21 saliva specimens with 0% false negatives. The proposed method can facilitate the diagnosis of COVID-19 and contribute to the prevention of early-stage transmission. English American Chemical Society Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR Article 10.1021/acs.analchem.3c02716 1 Analytical Chemistry, v.95, no.38, pp.14357 - 14364 Analytical Chemistry 95 38 14357 14364 N scie scopus 001074032800001 2-s2.0-85174334063 Chemistry, Analytical Chemistry Article ICVD IMPACT CHEMICAL-VAPOR-DEPOSITION