Park, Byeongho Bang, Seokyoung Hwang, Kyeong Seob Cha, Yeon Kyung Kwak, Jisung Tran, Na Ly Kim, Hyo-Suk Park, Subeen Oh, Seung Ja Im, Maesoon Chung, Sooyoung Kim, Jongbaeg Park, Tai Hyun Song, Hyun Seok Kim, Hong Nam Kim, Jae Hun 2024-01-19T09:00:31Z 2024-01-19T09:00:31Z 2023-08-02 2023-09 0935-9648 https://pubs.kist.re.kr/handle/201004/113349 An in vitro model, composed of the short-wavelength human opsins and rhodopsins, is created. Two types of photosensitive neural spheroids are transfected for selective reaction under bluish-purple and green lights. These are employed to two devices with intact neuron and neural-spheroid to study the interaction. By photostimulation, the photosensitive spheroid initiated photoactivation, and the signal generated from its body is transmitted to adjacent neural networks. Specifically, the signal traveled through the axon bundle in narrow gap from photosensitive spheroid to intact spheroid as an eye-to-brain model including optic nerve. The whole process with photosensitive spheroid is monitored by calcium ion detecting fluorescence images. The results of this study can be applied to examine vision restoration and novel photosensitive biological systems with spectral sensitivity. English WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Eye-Mimicked Neural Network Composed of Photosensitive Neural Spheroids with Human Opsin Proteins Article 10.1002/adma.202302996 1 Advanced Materials, v.35, no.35 Advanced Materials 35 35 N scie scopus 001027176500001 2-s2.0-85164750018 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article CONE PHOTORECEPTORS biological mimicking human opsin proteins neural networks photosensitive cells spheroids