Kim, Kyung Ho Seo, Sung Eun Kim, Jinyeong Park, Seon Joo An, Jai Eun Shin, Chan Jae Ryu, Choong-Min Lee, Sung Woon Nam, Ho Chul Yoon, Tae Ho Shin, Jong Cheol Kim, Yu Kyung Oh, Hanseul Hong, Jung Joo Kim, Brian N. Lee, Kyoung G. Song, Hyun Seok Lee, Sang Hun Kwon, Oh Seok 2024-01-19T09:00:57Z 2024-01-19T09:00:57Z 2023-06-08 2023-09 1616-301X https://pubs.kist.re.kr/handle/201004/113365 A molecular diagnosis of the respiratory syncytial virus (RSV) without bulky and expensive instrumentation is of great importance for the early detection and prevention in a fast-spreading pandemic. However, the current representative diagnostic methods have the limitation of being time-consuming, cost, the processing time for polymerase chain reaction (PCR), and inaccurate for lateral flow assay (LFA), representatively. Herein, an integrated photonic digital PCR (dPCR) is developed with high-velocity photonic scanner for in situ fluorescence detection by introducing the N-heterocyclic carbene self-assembled monolayer-based Au film to prevent the quenching effect. The on-site rapid molecular diagnostic platform shows the driving of 40 cycles in under 8 min and fluorescence scanning in under 7 min, resulting in a total analysis time within 15 min. In particular, the technology clearly demonstrates the classification of SARS-CoV-2 patients and healthy controls (99% in sensitivity, 98.6% in specificity, and 96.4% in accuracy with RdRp gene), comparing with standard RT-qPCR. This platform can be utilized for prompt point-of-care molecular diagnostics in early diagnosis and large-scale prevention of next pandemic spreading for upcoming infectious diseases and for the distinction diagnosis with other RSV. English John Wiley & Sons Ltd. Ultra-Fast Photonic Digital Polymerase Chain Reaction based on N-Heterocyclic Carbene Self-Assembled Monolayer Article 10.1002/adfm.202303728 1 Advanced Functional Materials, v.33, no.37 Advanced Functional Materials 33 37 N scie scopus 000992289100001 2-s2.0-85159876449 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article carbene chemistry dPCR PoCT portable devices respiratory syncytial virus