Lee, Shinae Heo, Seunga Park, Jihwan Heo, Jeongyun Kim, Sehoon You, Youngmin 2024-01-19T09:02:48Z 2024-01-19T09:02:48Z 2023-07-27 2023-08 2050-7518 https://pubs.kist.re.kr/handle/201004/113441 Glutathione (GSH) is an essential molecule that plays a pivotal role in maintaining intracellular redox homeostasis, as well as other physiological processes. However, the chemical mechanisms underlying the GSH-induced processes remain insufficiently understood due to the lack of appropriate detection tools. Fluorescence GSH imaging can serve as a useful principle for the rapid, convenient, and non-destructive detection of GSH in living organisms. In this study, we developed a fluorescent GSH probe based on a linear, homoleptic Au(i) complex with two 1,3-diphenylbenzimidazolium carbene ligands. The Au(i) complex produced a fluorescence turn-on response to GSH. Fluorescence GSH signaling was characterized with a short response time of a few seconds. The rapid response was attributed to the displacement of the carbene ligand with GSH, which involved a labile inner-sphere coordination interaction. Finally, we demonstrated the biological utility of our GSH probe by unambiguously discriminating between different GSH levels in normal and senescent preadipocytes. English Royal Society of Chemistry Glutathione displacement assay based on a fluorescent Au(i) complex Article 10.1039/d3tb00953j 1 Journal of Materials Chemistry B, v.11, no.31, pp.7344 - 7352 Journal of Materials Chemistry B 11 31 7344 7352 N scie scopus 001025037900001 Materials Science, Biomaterials Materials Science Article HIGHLY SELECTIVE DETECTION ON LUMINESCENT PROBE GENE-EXPRESSION RECENT PROGRESS SENESCENCE CYSTEINE GSH SENSOR CYS OLD