Lee, Hee Ju Zhang, Mi Doan, Thi Phuong Park, Eun-Jin Nghiem, Duc-Trong Pham, Ha-Thanh-Tung Pan, Cheol-Ho Oh, Won-Keun 2024-01-19T09:02:54Z 2024-01-19T09:02:54Z 2023-07-27 2023-08 0031-9422 https://pubs.kist.re.kr/handle/201004/113445 While screening senotherapeutics from natural products, seven undescribed chemicals, two syringylglycerol derivatives, two cyclopeptides, one tigliane analogue, and two chromone derivatives, as well as six known compounds were isolated from the stems of Limacia scandens. The structures of compounds were elucidated through spectroscopic data analysis, including 1D and 2D NMR, HRESIMS, and CD data. All compounds were tested in replicative senescent human dermal fibroblasts (HDFs) to determine their potential as senotherapeutic agents to specifically target senescent cells. One tigliane and two chromones derivatives showed senolytic activity, indicating that senescent cells were selectively removed. Especially, 2-{2-[(3'-O-beta-D-glucopyranosyl) phenyl]ethyl}chromone is expected to be a potential senotherapeutics by inducing HDF death, inhibiting the activity of senescence-associated beta-galactosidase (SA-beta-gal) and expressing senescence-associated secretory phenotype (SASP) factors. English Elsevier BV Chemical constituents with senolytic activity from the stems of Limacia scandens Article 10.1016/j.phytochem.2023.113740 1 Phytochemistry, v.212 Phytochemistry 212 N scie scopus 001017831700001 2-s2.0-85162744579 Biochemistry & Molecular Biology Plant Sciences Biochemistry & Molecular Biology Plant Sciences Article SENESCENT CELLS GLYCOSIDES GLUCOSIDE BETA Limacia scandens Menispermaceae Chromone derivatives Senolytic activity The activity of SA-beta-gal SASP factors