Tran, Phuong Hoang Nguyen Jung, Je Hyeong Ko, Ja Kyong Gong, Gyeongtaek Um, Youngsoon Lee, Sun-Mi 2024-01-19T09:03:05Z 2024-01-19T09:03:05Z 2023-07-06 2023-08 0960-1481 https://pubs.kist.re.kr/handle/201004/113453 The development of glucose/xylose co-fermenting Saccharomyces cerevisiae has improved bioethanol yield from lignocellulosic biomass, the most abundant and sustainable resource for net-zero production of fuels and chemicals. The co-production of value-added chemicals would further improve the economic feasibility of lignocellulosic bioethanol production. Here, we developed a glucose/xylose co-fermenting S. cerevisiae strain capable of co-producing polyhydroxybutyrate, a prominent biodegradable polymer, as an intracellularly accu-mulated co-product by introducing a polycistronic polyhydroxybutyrate biosynthetic pathway. The engineered strain accumulated polyhydroxybutyrate with a content of 64 mg/g DCW while maintaining extracellular pro-duction of ethanol with a high yield (0.43 g ethanol/g sugar). The co-production of ethanol and poly-hydroxybutyrate was then evaluated using various types of biomass, including sugarcane bagasse, silver grass, and even cardboard boxes. This study demonstrates the feasibility of co-production of bioethanol and value-added chemicals to maximize the values derivable from lignocellulosic biomass. English Pergamon Press Ltd. Co-production of ethanol and polyhydroxybutyrate from lignocellulosic biomass using an engineered Saccharomyces cerevisiae Article 10.1016/j.renene.2023.05.080 1 Renewable Energy, v.212, pp.601 - 611 Renewable Energy 212 601 611 N scie scopus 001010842300001 2-s2.0-85159883307 Green & Sustainable Science & Technology Energy & Fuels Science & Technology - Other Topics Energy & Fuels Article EXPRESSION VECTOR DELTA SEQUENCES PATHWAY XYLOSE BIOETHANOL STABILITY 2A Lignocellulosic biomass Bioethanol Biopolymer Polyhydroxybutyrate Polycistronic expression Saccharomyces cerevisiae