Chae, Uikyu Woo, Jiwan Cho, Yakdol Han, Jeong- Kyu Yang, Soo Hyun Yang, Esther Shin, Hyogeun Kim, Hyun Yu, Hyun- Yong Lee, C. Justin Cho, Il-Joo 2024-01-19T09:03:59Z 2024-01-19T09:03:59Z 2023-08-24 2023-07 0027-8424 https://pubs.kist.re.kr/handle/201004/113497 Real -time monitoring of various neurochemicals with high spatial resolution in mul-tiple brain regions in vivo can elucidate neural circuits related to various brain dis-eases. However, previous systems for monitoring neurochemicals have limitations in observing multiple neurochemicals without crosstalk in real time, and these methods cannot record electrical activity, which is essential for investigating neural circuits. Here, we present a real -time bimodal (RTBM) neural probe that uses monolithi-cally integrated biosensors and multiple shanks to study the connectivity of neural circuits by measuring multiple neurochemicals and electrical neural activity in real time. Using the RTBM probe, we demonstrate concurrent measurements of four neurochemicals-glucose, lactate, choline, and glutamate without cross-talking each other-and electrical activity in real time in vivo. Additionally, we show the func-tional connectivity between the medial prefrontal cortex and mediodorsal thalamus through the simultaneous measurement of chemical and electrical signals. We expect that our device will contribute to not only elucidating the role of neurochemicals in neural circuits related to brain functions but also developing drugs for various brain diseases related to neurochemicals. English National Academy of Sciences A neural probe for concurrent real-time measurement of multiple neurochemicals with electrophysiology in multiple brain regions in vivo Article 10.1073/pnas.2219231120 1 Proceedings of the National Academy of Sciences of the United States of America, v.120, no.28 Proceedings of the National Academy of Sciences of the United States of America 120 28 Y scie scopus 001038289500003 2-s2.0-85164001575 Multidisciplinary Sciences Science & Technology - Other Topics Article PUSH-PULL PERFUSION PREFRONTAL CORTEX GLUTAMATE GLUCOSE NEUROTRANSMITTER LACTATE ACETYLCHOLINE RELEASE RISK FLOW neural probe biosensor neurotransmitter MEMS neural circuit