Choi, Ju-Yeon Jeong, Miran Lee, Kijun Kim, Jin-Ok Lee, Wan Hee Park, InWha Kwon, Hak Cheol Choi, Jung-Hye 2024-01-19T09:04:09Z 2024-01-19T09:04:09Z 2023-08-11 2023-07 2076-3921 https://pubs.kist.re.kr/handle/201004/113507 Sedum middendorffianum Maxim (SMM) is a Korean endemic plant belonging to the Crassulaceae family. This study aimed to investigate the antitumor effects of the SMM extract on human ovarian cancer cells. Among five endemic plants grown in Korea, the SMM extract showed the most potent cytotoxicity in ovarian cancer cells and had little effect on normal ovarian surface epithelial cells. Furthermore, we revealed that the SMM extract dose-dependently induced apoptosis in human ovarian cancer A2780 and SKOV3 cells. The SMM extract markedly stimulated the activation of caspase-3/8, while the broad-spectrum caspase inhibitor and caspase-8 selective inhibitor significantly reversed SMM extract-induced apoptosis. In addition, the SMM extract significantly inhibited cell invasion and the expression levels of matrix metalloproteinase (MMP)-2 and MMP-9 in ovarian cancer cells. Notably, the SMM extract increased the generation of intracellular ROS, and pretreatment with antioxidant N-acetyl-L-cysteine (NAC) significantly suppressed SMM-induced cytotoxicity and anti-invasive activity. Moreover, NAC treatment reversed the SMM-induced inhibition of MMP-2/9 expression. Taken together, these data suggest that the SMM extract induces caspase-dependent apoptotic cell death and inhibits MMP-dependent invasion via ROS regulation. English MDPI AG Sedum middendorffianum Maxim Induces Apoptosis and Inhibits the Invasion of Human Ovarian Cancer Cells via Oxidative Stress Regulation Article 10.3390/antiox12071386 1 Antioxidants, v.12, no.7 Antioxidants 12 7 Y scie scopus 001034993500001 2-s2.0-85166026750 Biochemistry & Molecular Biology Chemistry, Medicinal Food Science & Technology Biochemistry & Molecular Biology Pharmacology & Pharmacy Food Science & Technology Article NADPH OXIDASE ANTITUMOR-ACTIVITY PLANTS BIOAVAILABILITY ANTIOXIDANT ACTIVATION EXPRESSION CASPASES PATHWAYS ROLES Sedum middendorffianum Maxim human ovarian cancer apoptosis invasion ROS MMP