KIM, HYO SUK Hyunju, Park Chang, Hyo Won Back, Ji hyun Lee Su Jin Park, Yae Eun Kim, Eun Hye Hong, Yeon sun kwak, gi jung Kwon, Ick Chan Lee, Ji Eun Lee, Yoon Se Kim, Sang Yoon Yang, Yoo soo Kim, Sun Hwa 2024-01-19T09:30:06Z 2024-01-19T09:30:06Z 2022-08-26 2023-07 2452-199X https://pubs.kist.re.kr/handle/201004/113610 Highly immunosuppressive tumor microenvironment containing various protumoral immune cells accelerates malignant transformation and treatment resistance. In particular, tumor-associated macrophages (TAMs), as the predominant infiltrated immune cells in a tumor, play a pivotal role in regulating the immunosuppressive tumor microenvironment. As a potential therapeutic strategy to counteract TAMs, here we explore an exosome-guided in situ direct reprogramming of tumor-supportive M2-polarized TAMs into tumor-attacking M1-type macrophages. Exosomes derived from M1-type macrophages (M1-Exo) promote a phenotypic switch from anti-inflammatory M2-like TAMs toward pro-inflammatory M1-type macrophages with high conversion efficiency. Reprogrammed M1 macrophages possessing protein-expression profiles similar to those of classically activated M1 macrophages display significantly increased phagocytic function and robust cross-presentation ability, potentiating antitumor immunity surrounding the tumor. Strikingly, these M1-Exo also lead to the conversion of human patient-derived TAMs into M1-like macrophages that highly express MHC class II, offering the clinical potential of autologous and allogeneic exosome-guided direct TAM reprogramming for arming macrophages to join the fight against cancer. ？ 2022 The Authors English Elsevier Exosome-guided direct reprogramming of tumor-associated macrophages from protumorigenic to antitumorigenic to fight cancer Article 10.1016/j.bioactmat.2022.07.021 1 Bioactive Materials, v.25, pp.527 - 540 Bioactive Materials 25 527 540 Y scie scopus 000973284900001 2-s2.0-85135517345 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article MICROENVIRONMENT MICRORNAS TARGETS MOUSE Tumor-associated macrophage Cancer therapy Direct conversion Exosome Tumor microenvironment