Lee, Seung Yeon Lee, Yujeong Choi, Nakwon Kim, Hong Nam Kim, Bumsang Sung, Jong Hwan 2024-01-19T09:32:18Z 2024-01-19T09:32:18Z 2023-03-10 2023-06 1976-0280 https://pubs.kist.re.kr/handle/201004/113722 The intestinal epithelium is a major barrier through which orally administered drugs must pass. The intestinal mucosa on the epithelium acts as an additional barrier that protects the intestinal cells from foreign substances, thereby interfering with drug delivery. Caco-2 based cell culture model is a standard in vitro model system for testing drug uptake. However, current in vitro models do not reflect the absorption mechanism of drugs accurately due to the absence of the mucus layer. Here, we developed a microfluidic gut-mucus chip using Caco-2 cells coated with mucin protein. It was confirmed that the mucin layer was maintained under flow conditions by Alcian blue/Periodic Acid Schiff (PAS) staining. In addition, the effect of mucosal layer on drug absorption in the flow environment was examined. Mucus-adhesive particles can be useful for delivery of drugs across the intestinal epithelium. We prepared mucus-adhesive and non-adhesive microparticles containing fluorescent molecules and compared the adhesion of these particles in flow condition. Mucus-coated Caco-2 cells provide a more physiologically realistic intestinal epithelial environment to study uptake processes of drugs released from the mucus-adhesive particles. We hope that the gut-mucus chip could potentially be used as novel and more accurate in vitro models of the intestine. English 한국바이오칩학회 Development of Gut-Mucus Chip for Intestinal Absorption Study Article 10.1007/s13206-023-00097-0 1 BioChip Journal, v.17, no.2, pp.230 - 243 BioChip Journal 17 2 230 243 N scie scopus kci ART002968234 000936708000002 2-s2.0-85148082243 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article IN-VITRO MODELS DRUG-DELIVERY GELATIN MUCOADHESIVE HYDROGELS CACO-2 MUCIN NANOPARTICLES DIFFUSION BARRIER Intestinal mucus Drug delivery system (DDS) Gut-on-a-chip