Kim, Sungbong Kim, Hyeong Ryeol Oh, Hyun Ju Lee, Kyu-Tae Um, Youngsoon Kim, Dong Wook Seo, Sang Woo Park, Chulhwan Kim, Seung Wook 2024-01-19T09:33:23Z 2024-01-19T09:33:23Z 2023-04-06 2023-05 1757-1693 https://pubs.kist.re.kr/handle/201004/113774 Xylose-rich undefined broth, extracted from the dilute acid pretreatment wastes of barley straw, serves as resourceful media for Acremonium chrysogenum M35 culture and production of cephalosporin C (CPC). Concentrating the extract with proper reprocessing enables to prepare various concentrations of xylose broth (2%-8%). The undefined xylose media were prepared for CPC production from A. chrysogenum M35 by the addition of other nutrients. Cell growth and CPC production were the most effective at 6% xylose and additional 2% glycerol, with maximum CPC production of 9.07 g/L after 6 days, which is higher production than that in defined media prepared with laboratory-level nutrients and reagents. Investigation of autotrophic and reverse trans-sulfuration pathways for cysteine synthesis, a limited element of three precursors for CPC synthesis, supports the enhanced CPC production in undefined media. Abundance of xylose ensures a maintained NADPH concentration required for sulfate reduction and synthesis of amino sulfide such as cysteine. Cystathionine-gamma-lyase activity profiling indicated more efficient biosynthesis in undefined media than in other cultures use glycerol and glucose, and the biosynthesis pathway of CPC production by the cephalosporin gene cluster (i.e. pcbC and cefG genes) was investigated. The process using undefined xylose media was designed, and process simulation program confirmed our results. English Wiley-VCH Verlag Undefined xylose media extracted from biorefinery waste for enhanced and eco-friendly production of cephalosporin C by Acremonium chrysogenum M35 Article 10.1111/gcbb.13049 1 GCB Bioenergy, v.15, no.5, pp.731 - 742 GCB Bioenergy 15 5 731 742 Y scie scopus 000948514400001 2-s2.0-85150630587 Agronomy Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article BUTYRIC-ACID PRODUCTION BARLEY STRAW CEFG GENE BIOSYNTHESIS FERMENTATION EXPRESSION PENICILLIN ENCODES biorefinery cephalosporin C dilute acid pretreatment echo-friendly process waste reprocess xylose fermentation