Blaudszun, Andre-Rene Kim, Woo Jun Um, Wooram Yoon, Hong Yeol Shim, Man Kyu Kim, Kwangmeyung 2024-01-19T09:34:00Z 2024-01-19T09:34:00Z 2023-06-01 2023-04 1999-4923 https://pubs.kist.re.kr/handle/201004/113802 Adoptive cell transfer (ACT) has shown remarkable therapeutic efficacy against blood cancers such as leukemia and lymphomas, but its effect is still limited due to the lack of well-defined antigens expressed by aberrant cells within tumors, the insufficient trafficking of administered T cells to the tumor sites, as well as immunosuppression induced by the tumor microenvironment (TME). In this study, we propose the adoptive transfer of photosensitizer (PS)-loaded cytotoxic T cells for a combinational photodynamic and cancer immunotherapy. Temoporfin (Foscan((R))), a clinically applicable porphyrin derivative, was loaded into OT-1 cells (PS-OT-1 cells). The PS-OT-1 cells efficiently produced a large amount of reactive oxygen species (ROS) under visible light irradiation in a culture; importantly, the combinational photodynamic therapy (PDT) and ACT with PS-OT-1 cells induced significant cytotoxicity compared to ACT alone with unloaded OT-1 cells. In murine lymphoma models, intravenously injected PS-OT-1 cells significantly inhibited tumor growth compared to unloaded OT-1 cells when the tumor tissues were locally irradiated with visible light. Collectively, this study suggests that combinational PDT and ACT mediated by PS-OT-1 cells provides a new approach for effective cancer immunotherapy. English Multidisciplinary Digital Publishing Institute (MDPI) Adoptive Transfer of Photosensitizer-Loaded Cytotoxic T Cells for Combinational Photodynamic Therapy and Cancer Immuno-Therapy Article 10.3390/pharmaceutics15041295 1 Pharmaceutics, v.15, no.4 Pharmaceutics 15 4 Y scie scopus 000977745600001 2-s2.0-85154543031 Pharmacology & Pharmacy Pharmacology & Pharmacy Article KILLER-CELLS RICIN cancer immunotherapy photodynamic therapy combination therapy adoptive T cell therapy cell-mediated drug delivery