Kim, Youngwoong Kim, Jaehoon An, Jong Min Park, Chul-Kee Kim, Dokyoung 2024-01-19T09:34:35Z 2024-01-19T09:34:35Z 2023-04-20 2023-04 2379-3694 https://pubs.kist.re.kr/handle/201004/113831 Fluorescence-guided surgery (FSG) is a surgical method to selectively visualize the tumor site using fluorescent materials with instrumental setups in the operation rooms. It has been widely used in the surgery of brain tumors, such as glioblastoma (GBM), which is difficult to distinguish from normal tissue. Although FSG is crucial for GBM surgery, the commercially available fluorescent materials for FSG have shown serious adverse effects. To satisfy the clinical demand, we recently reported reaction-based fluorescent probes based on a 4-chloro-7-nitrobenzofurazan (NBD) fluorophore that can detect cysteine (Cys) and homocysteine (Hcy), a biomarker of GBM, and their applications for the GBM diagnosis and FSG. However, our probes have cellular toxicity issues arising from the leaving group (LG) that is generated after the reaction of the fluorescent probe and the analytes. In this study, we disclosed a nontoxic fluorescent probe for sensing biothiols and their clinical applications for real-time human glioblastoma visualization. Systematic toxicity analysis of several LGs was conducted on several cell lines. Among the LGs, 2hydroxy-pyridine showed negligible toxicity, and its fluorescent probe derivative (named NPO-o-Pyr) showed high specificity and sensitivity (LOD: 0.071 ppm for Cys; 0.189 ppm for Hcy), a fast response time (<5 min) to Cys and Hcy, and high biocompatibility. In addition, NPO-o-Pyr can significantly detect the GBM site both in actual clinical samples as well as in the GBM-xenografted mouse model. We are confident that NPO-o-Pyr will become a new substitute in FSG due to its capability to overcome the limitations of the current fluorescent probes. English AMER CHEMICAL SOC All-Nontoxic Fluorescent Probe for Biothiols and Its Clinical Applications for Real-Time Glioblastoma Visualization Article 10.1021/acssensors.3c00004 1 ACS Sensors, v.8, no.4, pp.1723 - 1732 ACS Sensors 8 4 1723 1732 N scie scopus 000959734600001 2-s2.0-85151388320 Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Chemistry Science & Technology - Other Topics Article; Early Access SELECTIVE DETECTION SURGERY THERAPY PERSPECTIVES MECHANISM RESECTION CYSTEINE fluorescent probe reaction-based probe biothiol sensing glioblastoma image-guided surgery