Kim, Hong-Rae Bong, Ji-Hong Kang, Min-Jung Shim, Won-Bo Lee, Jeong-Soo Pyun, Jae-Chul 2024-01-19T10:00:34Z 2024-01-19T10:00:34Z 2023-02-17 2023-04 0039-9140 https://pubs.kist.re.kr/handle/201004/113887 A one-step immunoassay based on filtration was presented, which used microbeads for target analyte detection and filters with appropriate pore sizes to distinguish the complexity of target analyte and microbeads. For effective bacterial detection, the microbead size and the filter's pore size must be optimized. The optimal con-centrations of the enzyme (urease) and antibody were determined at the maximum absorbance change, that is, the maximum pH change. The pH change was measured using a field-effect transistor (FET). The correlation between pH change and threshold voltage was estimated to be 21.7 mV/pH, and the correlation between pH change and the source-drain current was estimated to be-379 nA/pH. For the one-step immunoassay, antibodies against target bacteria were isolated from horse serum by filtration, and these antibodies were estimated to have a sufficiently high specificity to overcome cross-reactivity among five types of food poisoning-related bacteria: Escherichia coli O157, Salmonella typhimurium, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. Finally, the FET-based one-step immunoassay was demonstrated for five types of food poisoning-related bacteria in human serum. English Elsevier BV One-step immunoassay based on filtration for detection of food poisoning-related bacteria Article 10.1016/j.talanta.2022.124203 1 Talanta, v.255 Talanta 255 N scie scopus 000918700700001 2-s2.0-85144622848 Chemistry, Analytical Chemistry Article INFLUENZA-A H1N1 ESCHERICHIA-COLI OUTER-MEMBRANE LAYER One-step immunoassay based on filtration Field-effect transistor Food poisoning-related bacteria Urease