Nam, Keonwook Kim, Young Min Choi, Inseok Han, Hwa Seung Kim, Taehyung Choi, Ki Young Roh, Young Hoon 2024-01-19T10:00:45Z 2024-01-19T10:00:45Z 2023-11-15 2023-03 0168-3659 https://pubs.kist.re.kr/handle/201004/113892 Despite the vast interest in utilizing rolling circle amplification (RCA)-based DNA networks for bioapplications, precise control of the mechanical and physicochemical properties is highly challenging. To address this concern, we aimed to develop ultrasoft self-supporting polymerized DNA networks (pDNets) of variable crystallinities to manipulate sequence-mediated drug release efficiency. A controlled ratio of the inorganic magnesium pyro-phosphate (MgPPi) crystal to the organic polymeric DNA resulted in the synthesis of pDNets of various nano -porosities. The number of crystal microstructures influencing drug localization and release pattern and the tunable mechanical properties influencing injectability and structural stability under physiological conditions were investigated. The pDNets exhibited ultrasoft properties with Young's moduli of 0.06-0.54 Pa; approxi-mately 9-fold differences in mechanical properties were obtained by varying the degree of crystallinity. With functional DNA sequences, the developed platforms showed pH stimuli-responsive drug release profiles of the dynamic DNA structures and aptamer-specific cell target adhesion efficiency. Analyses of controlled delivery of anticancer therapeutics in vitro and in vivo revealed crystallinity-dependent antitumor efficacy without side effects. This strategy provides an effective one-pot enzymatic polymerization methodology and a favorable microenvironment for a three-dimensional DNA network based on demand-localized drug delivery. English Elsevier BV Crystallinity-tuned ultrasoft polymeric DNA networks for controlled release of anticancer drugs Article 10.1016/j.jconrel.2023.01.056 1 Journal of Controlled Release, v.355, pp.7 - 17 Journal of Controlled Release 355 7 17 N scie scopus 000969005500001 2-s2.0-85147256277 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article ROLLING CIRCLE AMPLIFICATION IN-SITU DELIVERY NANOMATERIALS NANOCARRIERS PARTICLES DNA hydrogel Dynamic DNA structure Injectable hydrogel Controlled drug delivery cancer therapy Rolling circle amplification