Roh, Soonjong Lee, Kangwon Jung, Youngmee Yoo, Jin 2024-01-19T10:01:15Z 2024-01-19T10:01:15Z 2023-04-20 2023-03 2296-8016 https://pubs.kist.re.kr/handle/201004/113912 Surface modification of polymeric scaffolds for biomedical applications is a versatile and widely used method to improve interactions between scaffolds and cells. Specifically, chemical immobilization of proteins to polymeric scaffolds provides significant advantages such as stable and biocompatible properties, however, it generally requires expensive instruments or complex steps. In this study, the facile method is presented that changes poly (l-lactide-co-caprolactone) electrospun nanofibrous membranes to be cell-friendly and bioactive by chemical immobilization of proteins onto membranes. The model protein, bovine serum albumin was bound onto the nanofibrous membranes via aminolysis and subsequent covalent grafting. The surface modification effects of the nanofibrous membranes including surface morphologies, mechanical properties, and wettability were investigated. In addition, in vitro studies have demonstrated that adhesion and proliferation of human dermal fibroblasts are promoted in protein-immobilized membranes compared to bare nanofibrous membranes. English Frontiers Media S.A. Facile method for immobilization of protein on elastic nanofibrous polymer membranes Article 10.3389/fmats.2023.1141154 1 Frontiers in Materials, v.10 Frontiers in Materials 10 Y scie scopus 000964926400001 Materials Science, Multidisciplinary Materials Science Article SURFACE MODIFICATION CELL-ADHESION TISSUE POLY(L-LACTIDE-CO-EPSILON-CAPROLACTONE) AMINOLYSIS SCAFFOLD protein immobilization electrospinning nanofibers Poly(l-lactic-co-epsilon-caprolactone) surface modifcation