Pham, Ha-Thanh-Tung Park, Eun-Jin Ryu, Byeol Lee, Hee-Ju Doan, Thi-Phuong Cho, Hyo-Moon Pham, Thi-Linh-Giang Oh, Won-Keun 2024-01-19T10:30:51Z 2024-01-19T10:30:51Z 2023-02-03 2023-01 0031-9422 https://pubs.kist.re.kr/handle/201004/114137 Chemical investigation of the plant Gymnema latifolium led to the isolation of seven undescribed 23-glycosyl oleanane triterpenoids, gymlatinosides GLF1-GLF7, and two known compounds, gymnemosides D and E. The structures of the isolated compounds were elucidated using diverse spectroscopic methods. The extract of G. latifolium and all isolated compounds significantly enhanced 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) uptake into 3T3-L1 adipocytes at 20 mu M. Among them, gymlatinosides GLF2 and gymlatinosides GLF4 showed particularly potent stimulatory effects on glucose uptake in a dose-dependent manner. Further investigation revealed that gymlatinosides GLF2 at 20 mu M upregulated the expression of phosphorylated AMPK (p-AMPK). The results suggested that gymlatinosides GLF2 may enhance glucose uptake via regulating the AMPK signaling pathway. English Elsevier BV Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium Article 10.1016/j.phytochem.2022.113513 1 Phytochemistry, v.205 Phytochemistry 205 N scie scopus 000904902200008 2-s2.0-85142184385 Biochemistry & Molecular Biology Plant Sciences Biochemistry & Molecular Biology Plant Sciences Article GLUCOSE-UPTAKE LEAVES AMPK GLYCOSIDES Gymnema latifolium Apocynaceae 23-Glycosyl oleanane glycosides Insulin mimetics Glucose uptake Antidiabetic compound AMPK signaling Pathway