Lee, Hae-Ri Kim, Soo Shin, Sungho Jeong, Seon-Yeong Lee, Dae-Won Lim, Sun-Ung Kang, Ji Yeon Son, Mi-Young Lee, Cheolju Yu, Kyung-Rok Kim, Myungshin Oh, Il-Hoan 2024-01-19T10:31:10Z 2024-01-19T10:31:10Z 2023-01-19 2023-01 1661-6596 https://pubs.kist.re.kr/handle/201004/114153 Mesenchymal stromal cells derived from induced pluripotent stem cells (iMSCs) have been proposed as alternative sources of primary MSCs with various advantages for cell therapeutic trials. However, precise evaluation of the differences between iMSCs and primary MSCs is lacking due to individual variations in the donor cells, which obscure direct comparisons between the two. In this study, we generated donor-matched iMSCs from individual bone marrow-derived MSCs and directly compared their cell-autonomous and paracrine therapeutic effects. We found that the transition from primary MSCs to iMSCs is accompanied by a functional shift towards higher proliferative activity, with variations in differentiation potential in a donor cell-dependent manner. The transition from MSCs to iMSCs was associated with common changes in transcriptomic and proteomic profiles beyond the variations of their individual donors, revealing expression patterns unique for the iMSCs. These iMSC-specific patterns were characterized by a shift in cell fate towards a pericyte-like state and enhanced secretion of paracrine cytokine/growth factors. Accordingly, iMSCs exhibited higher support for the self-renewing expansion of primitive hematopoietic progenitors and more potent immune suppression of allogenic immune responses than MSCs. Our study suggests that iMSCs represent a separate entity of MSCs with unique therapeutic potential distinct from their parental MSCs, but points to the need for iMSC characterization in the individual basis. English Multidisciplinary Digital Publishing Institute (MDPI) iPSC-Derived MSCs Are a Distinct Entity of MSCs with Higher Therapeutic Potential than Their Donor-Matched Parental MSCs Article 10.3390/ijms24010881 1 International Journal of Molecular Sciences, v.24, no.1 International Journal of Molecular Sciences 24 1 Y scie scopus 000908703500001 2-s2.0-85145920267 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article MESENCHYMAL STEM-CELLS STROMAL CELLS CD34(+) CELLS SELF-RENEWAL NICHE GENERATION INHIBIT TISSUE iPSC mesenchymal stromal cells(MSCs) cell therapy cell autologous cell differentiation