Kim, Bong Kyun Lee, Sang-A Park, Minju Jeon, Eui Ju Kim, Mi Jung Kim, Jung Min Kim, Heesuk Jung, Seungwon Kim, Sang Kyung 2024-01-19T10:32:45Z 2024-01-19T10:32:45Z 2022-12-22 2022-12 1936-0851 https://pubs.kist.re.kr/handle/201004/114228 As the turnaround time of diagnosis becomes important, there is an increasing demand for rapid, point-of-care testing (POCT) based on polymerase chain reaction (PCR), the most reliable diagnostic tool. Although optical components in real-time PCR (qPCR) have quickly become compact and economical, conventional PCR instruments still require bulky thermal systems, making it difficult to meet emerging needs. Photonic PCR, which utilizes photothermal nanomaterials as heating elements, is a promising platform for POCT as it reduces power consumption and process time. Here, we develop a photonic qPCR platform using hydrogel microparticles. Microparticles consisting of hydrogel matrixes containing photothermal nanomaterials and primers are dubbed photothermal primer-immobilized networks (pPINs). Reduced graphene oxide is selected as the most suitable photothermal nanomaterial to generate heat in pPIN due to its superior light-to-heat conversion efficiency. The photothermal reaction volume of 100 nL (predefined by the pPIN dimensions) provides fast heating and cooling rates of 22.0 +/- 3.0 and 23.5 +/- 2.6 degrees C s-1, respectively, enabling ultrafast qPCR within 5 min only with optical components. The microparticle-based photonic qPCR facilitates multiplex assays by loading multiple encoded pPIN microparticles in a single reaction. As a proof of concept, four-plex pPIN qPCR for bacterial discrimination are successfully demonstrated. English American Chemical Society Ultrafast Real-Time PCR in Photothermal Microparticles Article 10.1021/acsnano.2c07017 1 ACS Nano, v.16, no.12, pp.20533 - 20544 ACS Nano 16 12 20533 20544 N scie scopus 000893463200001 2-s2.0-85143648027 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article POLYMERASE-CHAIN-REACTION GOLD SYSTEM photonic PCR hydrogel reduced graphene oxide (rGO) real-time PCR multiplex assay bacteria