Lee, Chang Kyu Jung, Jaeyong Kim, Hong-Rae Bong, Ji-Hong Kim, Tae-Hun Park, Jun-Hee Kwon, Soonil Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T10:33:21Z 2024-01-19T10:33:21Z 2022-11-04 2022-12 0003-2654 https://pubs.kist.re.kr/handle/201004/114251 A one-step immunoassay was developed for five types of food-poisoning-related bacteria using a switching peptide and antibodies isolated from unimmunized horse serum. The one-step immunoassay involves mixing samples and reagents in a homogeneous solution without any washing steps. In this work, a one-step immunoassay configuration was developed using isolated antibodies labelled with an organic fluorescence quencher and a switching-peptide labelled with a fluorescent dye. The fluorescence-labelled switching-peptide was bound to the antigen-binding site of the isolated antibodies before binding to the bacteria (no fluorescence signal), and the switching-peptide dissociated from the antibodies as soon as they bound to the bacteria (fluorescence signal turns on). By quantifying the generated fluorescence signal, the one-step immunoassay presented here allows microbial detection without any washing step. English Royal Society of Chemistry One-step immunoassay for the detection of food-poisoning related bacteria using a switching peptide Article 10.1039/d2an00940d 1 Analyst, v.147, no.23, pp.5363 - 5371 Analyst 147 23 5363 5371 N scie scopus 000871543400001 Chemistry, Analytical Chemistry Article; Early Access SURFACE-PLASMON RESONANCE FLUORESCENCE IMMUNOASSAY SALMONELLA-TYPHIMURIUM ESCHERICHIA-COLI OUTER-MEMBRANE HUMAN SERUM LABEL-FREE LIPOPOLYSACCHARIDE ANTIBODIES