Lee, Geonhee Oh Yuna Nam, Jung Tae Ji, Seulgi Jang, A-Rang Jeong, Du Won Kang, MinSoung Lee, Sun Sook Chae, Soosang Cho, Donghwi Hwang, Jun Yeon Lee, Kyungeun Lee, Jeong-O 2024-01-19T10:33:32Z 2024-01-19T10:33:32Z 2022-10-20 2022-12 0957-4484 https://pubs.kist.re.kr/handle/201004/114260 In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z-axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate. English Institute of Physics Publishing Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology Article 10.1088/1361-6528/ac9143 1 Nanotechnology, v.33, no.50 Nanotechnology 33 50 Y scie scopus 000863503200001 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Science & Technology - Other Topics Materials Science Physics Article ELECTRON ULTRASTRUCTURE WETTABILITY MORPHOLOGY MECHANISM ADHESION graphene polydimethylsiloxane (PDMS) scanning electron microscope (SEM) correlative light and electron microscopy (CLEM)