Jeon, Jongbum Kim, Ki-Tae Choi, Jaeyoung Cheong, Kyeongchae Ko, Jaeho Choi, Gobong Lee, Hyunjun Lee, Gir-Won Park, Sook-Young Kim, Seongbeom Kim, Sun Tae Min, Cheol Woo Kang, Seogchan Lee, Yong-Hwan 2024-01-19T10:33:55Z 2024-01-19T10:33:55Z 2022-04-03 2022-12 1547-6286 https://pubs.kist.re.kr/handle/201004/114277 Alternative splicing (AS) contributes to diversifying and regulating cellular responses to environmental conditions and developmental cues by differentially producing multiple mRNA and protein isoforms from a single gene. Previous studies on AS in pathogenic fungi focused on profiling AS isoforms under a limited number of conditions. We analysed AS profiles in the rice blast fungus Magnaporthe oryzae, a global threat to rice production, using high-quality transcriptome data representing its vegetative growth (mycelia) and multiple host infection stages. We identified 4,270 AS isoforms derived from 2,413 genes, including 499 genes presumably regulated by infection-specific AS. AS appears to increase during infection, with 32.7% of the AS isoforms being produced during infection but absent in mycelia. Analysis of the isoforms observed at each infection stage showed that 636 AS isoforms were more abundant than corresponding annotated mRNAs, especially after initial hyphal penetration into host cell. Many such dominant isoforms were predicted to encode regulatory proteins such as transcription factors and phospho-transferases. We also identified the genes encoding distinct proteins via AS and confirmed the translation of some isoforms via a proteomic analysis, suggesting potential AS-mediated neo-functionalization of some genes during infection. Comprehensive profiling of the pattern of genome-wide AS during multiple stages of rice-M. oryzae interaction established a foundational resource that will help investigate the role and regulation of AS during rice infection. English Landes Bioscience Alternative splicing diversifies the transcriptome and proteome of the rice blast fungus during host infection Article 10.1080/15476286.2022.2043040 1 RNA Biology, v.19, no.1, pp.373 - 385 RNA Biology 19 1 373 385 Y scie scopus 000771520500001 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article GENE-EXPRESSION SR PROTEINS COMPLEXITY SEQUENCE ORYZAE LANDSCAPE STRINGTIE GENOMICS INSIGHTS BIOLOGY Alternative splicing functional diversification magnaporthe oryzae pathogenicity proteome rice transcriptome