Bae, Yunji Kim, Taeyoung Park, Nojune Choi, Sangho Yi, Dongkeun Soto, Silvia Zamora, Nelson Kim, Sunam Yang, Minhye 2024-01-19T10:34:16Z 2024-01-19T10:34:16Z 2022-12-01 2022-11 2072-6643 https://pubs.kist.re.kr/handle/201004/114297 The genus Daphnopsis has been traditionally used as a purgative, diuretic, stimulant, and psoriasis treatment. In this study, the anti-AD (atopic dermatitis) activities of the Daphnopsis costaricensis EtOH extract (DCE) were investigated in an oxazolone (OX)-induced mouse model of AD, and the anti-inflammatory effects of its active compounds were confirmed in PI-sensitized or IgE/DNP-BSA-sensitized RBL-2H3 cells. DCE improved the symptoms of OX-induced inflammatory dermatitis (swelling, erythema, and increased ear thickening) in OX-induced BALB/c mice ears and reduced epidermal thickness and mast cell infiltration. Eleven flavonoid compounds were isolated from DCE, and two compounds (7,8-dimethoxyflavone and 7,2'-dimethoxyflavone) significantly inhibited IL-4 overexpression in PI-induced RBL-2H3 cells and mast cell degranulation in IgE + DNP-BSA-induced RBL-2H3 cells. Our study indicates that DCE and two compounds (7,8-dimethoxyflavone and 7,2'-dimethoxyflavone) might effectively improve inflammatory and atopic skin symptoms. English Multidisciplinary Digital Publishing Institute (MDPI) Ameliorative Effects of Daphnopsis costaricensis Extract against Oxazolone-Induced Atopic Dermatitis-like Lesions in BALB/c Mice Article 10.3390/nu14214521 1 Nutrients, v.14, no.21 Nutrients 14 21 Y scie scopus 000881471100001 Nutrition & Dietetics Nutrition & Dietetics Article C-13 NMR DATA IN-VITRO FLAVONOIDS H-1 INHIBITORS CYTOKINES ALLERGY Daphnopsis costariensis atopic dermatitis anti-inflammatory 7,8-dimethoxyflavone 7,2 &apos -dimethoxyflavone interleukin 4 beta-hexosaminidase