Noh, Joo-Yoon Kim, Moon-Ju Yun, Tae Gyeong Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T11:00:39Z 2024-01-19T11:00:39Z 2022-10-20 2022-11 0003-2654 https://pubs.kist.re.kr/handle/201004/114419 Short and medium chain acylcarnitines have been used for the diagnosis of various fatty acid oxidation and organic acid disorders. This report presents a multiplex and quantitative analysis of acylcarnitines using MALDI-TOF MS based on a parylene matrix chip. The parylene matrix chip was fabricated by the deposition of a nanoporous film of parylene on an organic matrix array, which reduced the number of mass peaks from the organic matrix in the low m/z range. Quantitative analysis was possible using the parylene matrix chip because of the formation of nano-sized sample crystals on the nanoporous parylene film. Seven acylcarnitines were quantitatively analyzed using the chip; the method detection range included the cut-off values for metabolic disorders. The seven acylcarnitines of different concentrations were simultaneously detected using the parylene matrix chip and the interference from the mixed carnitines was estimated. Real l-carnitine (C0) samples were analyzed using serial dilution, and the recoveries were calculated by comparisons with a standard curve. English Royal Society of Chemistry Simultaneous analysis of acylcarnitines using MALDI-TOF mass spectrometry based on a parylene matrix chip Article 10.1039/d2an01199a 1 Analyst, v.147, no.22, pp.5044 - 5053 Analyst 147 22 5044 5053 N scie scopus 000863892300001 Chemistry, Analytical Chemistry Article LASER-DESORPTION IONIZATION SMALL-MOLECULE ANALYSIS FATTY-ACID