Jeong, In Hwa Kim, Ho Kyeong Kim, Hye Ri Kim, Jungbae Kim, Byoung Chan 2024-01-19T11:00:51Z 2024-01-19T11:00:51Z 2022-09-15 2022-11 1618-2642 https://pubs.kist.re.kr/handle/201004/114427 Airborne microbes can rapidly spread and cause various infectious diseases worldwide. This necessitates the determination of a fast and highly sensitive detection method. There have been no studies on receptors targeting Citrobacter braakii (C. braakii), a pathogenic bacterium which can exist in the air. In this study, we rapidly isolate an aptamer, a nucleic acid molecule that can specifically bind to C. braakii by centrifugation-based partitioning method (CBPM) reported previously by our groups as omitting the repeated rounds of binding incubation, separation, and amplification that are indispensable for SELEX. The binding affinity and specificity of isolated aptamers are checked using bacteria in liquid culture and recollection solution from aerosolized bacteria. Recollection solutions of the recovered bacteria are obtained by nebulizing, drying, and recapturing with a biosampler. The CB-5 aptamer shows high affinity and specificity for C. braakii (K-d: 16.42 in liquid culture and 26.91 nM in recollection from aerosolized sample). Our results indicate the current protocol can be employed for the rapid development of reliable diagnostic receptors targeting airborne bacteria. English Springer Verlag Development of aptamers for rapid airborne bacteria detection Article 10.1007/s00216-022-04308-9 1 Analytical and Bioanalytical Chemistry, v.414, no.27, pp.7763 - 7771 Analytical and Bioanalytical Chemistry 414 27 7763 7771 N scie scopus 000850777600001 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article WASTE-WATER CELL-SELEX TECHNOLOGY Aptamer Non-SELEX Citrobacter braakii Bioreceptor Airborne bacteria