Jo, Young-Woo Park, Inkuk Yoo, Kyusang Woo, Hyun-Young Kim, Ye Lynne Kim, Yea-Eun Kim, Ji-Hoon Kong, Young-Yun 2024-01-19T11:00:57Z 2024-01-19T11:00:57Z 2022-09-02 2022-11 1066-5099 https://pubs.kist.re.kr/handle/201004/114432 Myogenic progenitors (MPs) generate myocytes that fuse to form myofibers during skeletal muscle development while maintaining the progenitor pool, which is crucial for generating sufficient muscle. Notch signaling has been known to reserve a population of embryonic MPs during primary myogenesis by promoting cell cycle exit and suppressing premature differentiation. However, the roles of individual Notch receptors (Notch1-4) during embryonic/fetal myogenesis are still elusive. In this study, we found that Notch1 and Notch2, which exhibit the highest structural similarity among Notch receptors, maintain the MP population by distinct mechanisms: Notch1 induces cell cycle exit and Notch2 suppresses premature differentiation. Moreover, genetic and cell culture studies showed that Notch1 and Notch2 signaling in MPs are distinctively activated by interacting with Notch ligand-expressing myofibers and MP-lineage cells, respectively. These results suggest that through different activation modes, Notch1 and Notch2 distinctively and cooperatively maintain MP population during fetal myogenesis for proper muscle development. English AlphaMed Press Inc Notch1 and Notch2 Signaling Exclusively but Cooperatively Maintain Fetal Myogenic Progenitors Article 10.1093/stmcls/sxac056 1 Stem Cells, v.40, no.11, pp.1031 - 1042 Stem Cells 40 11 1031 1042 N scie scopus 000842623900001 Cell & Tissue Engineering Biotechnology & Applied Microbiology Oncology Cell Biology Hematology Cell Biology Biotechnology & Applied Microbiology Oncology Hematology Article; Early Access MUSCLE STEM-CELLS SELF-RENEWAL DIFFERENTIATION MAINTENANCE QUIESCENT PROMOTES DOMAINS NICHE PAX7 skeletal muscle fetal myogenesis myogenic progenitor notch signaling stem cell maintenance