Jeong, Ahyeon Cho, Yena Cho, Minkyeong Bae, Gyu-Un Song, Dae-Geun Kim, Su-Nam Kim, Yong Kee 2024-01-19T11:01:33Z 2024-01-19T11:01:33Z 2022-11-10 2022-10 1661-6596 https://pubs.kist.re.kr/handle/201004/114461 Protein arginine methyltransferase 7 (PRMT7) regulates various cellular responses, including gene expression, cell migration, stress responses, and stemness. In this study, we investigated the biological role of PRMT7 in cell cycle progression and DNA damage response (DDR) by inhibiting PRMT7 activity with either SGC8158 treatment or its specific siRNA transfection. Suppression of PRMT7 caused cell cycle arrest at the G(1) phase, resulting from the stabilization and subsequent accumulation of p21 protein. In addition, PRMT7 activity is closely associated with DNA repair pathways, including both homologous recombination and non-homologous end-joining. Interestingly, SGC8158, in combination with doxorubicin, led to a synergistic increase in both DNA damage and cytotoxicity in MCF7 cells. Taken together, our data demonstrate that PRMT7 is a critical modulator of cell growth and DDR, indicating that it is a promising target for cancer treatment. English Multidisciplinary Digital Publishing Institute (MDPI) PRMT7 Inhibitor SGC8158 Enhances Doxorubicin-Induced DNA Damage and Its Cytotoxicity Article 10.3390/ijms232012323 1 International Journal of Molecular Sciences, v.23, no.20 International Journal of Molecular Sciences 23 20 Y scie scopus 000873240100001 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article METHYLTRANSFERASE 7 PRMT7 ARGININE METHYLATION PHOSPHORYLATION MECHANISM CANCER REPAIR BRCA2 53BP1 protein arginine methyltransferase 7 SGC8158 DNA damage response senescence cell cycle