Yun, Tae Gyeong Kim, Moon-Ju Noh, Joo-Yoon Song, Zhiquan Kim, Younghun Kang, Min-Jung Park, Hyung-Ho Pyun, Jae-Chul 2024-01-19T11:01:56Z 2024-01-19T11:01:56Z 2022-10-27 2022-10 2574-0970 https://pubs.kist.re.kr/handle/201004/114477 The SiO2 aerogel has a porous network of spherical silica particles with an average size of 3-5 nm, which provides an extremely high surface area. Herein, SiO2 aerogel was synthesized using the sol-gel method with sodium silicate as a precursor, and the SiO2 aerogel was applied to matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS) as an inorganic matrix for quantitative analysis. In this work, a combi-matrix was prepared by mixing the SiO2 aerogel and a conventional organic matrix (CHCA) for quantitative analysis of analytes at an m/z ratio of less than 500. Using the combi-matrix based on the SiO2 aerogel with an extremely high porosity, analyte ionization was performed without the mass peaks of the organic matrix (CHCA) for MALDI-MS. The optimal ratio between the SiO2 aerogel and the organic matrix was determined to eliminate the mass peaks from the organic matrix and maximize the signal-to-noise ratio (S/N ratio) of the analytes. Quantitative analysis was performed using the optimal combi-matrix system (a mixture of SiO2 aerogel and organic matrix (CHCA)). The homogeneous and dense distribution of analytes was demonstrated using fluorescence imaging, and the spot-to-spot and shot-to-shot reproducibility was estimated using the SiO2 aerogel. Finally, LDI-MS based on the combi-matrix was applied for the quantitative analysis of a biomarker for colon cancer, dodecanoyl-L-carnitine (DC). Using real samples from healthy volunteers and patients, a medical diagnosis of colon cancer was demonstrated using a combi-matrix. English American Chemical Society Laser-Assisted Desorption/Ionization Mass Spectrometry Using Nanoporous SiO2 Aerogels for the Diagnosis of Colon Cancer Article 10.1021/acsanm.2c03084 1 ACS Applied Nano Materials, v.5, no.10, pp.14798 - 14810 ACS Applied Nano Materials 5 10 14798 14810 N scie scopus 000868855200001 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article QUANTITATIVE-ANALYSIS DESORPTION-IONIZATION SODIUM-SILICATE SMALL MOLECULES DOSE-RESPONSE SOLID-MATRIX ACID ACYLCARNITINES PROTEINS SAMPLES dodecanoyl-L-carnitine (DC) colon cancer MALDI-MS SiO2 aerogel combi-matrix