Kim, Hong-Rae Bong, Ji-Hong Kim, Tae-Hun Shin, Seung-Shick Kang, Min-Jung Shim, Won-Bo Lee, Do Young Son, Dong Hee Pyun, Jae-Chul 2024-01-19T11:30:12Z 2024-01-19T11:30:12Z 2022-08-11 2022-09 1976-0280 https://pubs.kist.re.kr/handle/201004/114726 One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays. English 한국바이오칩학회 One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher Article 10.1007/s13206-022-00076-x 1 BioChip Journal, v.16, no.3, pp.334 - 341 BioChip Journal 16 3 334 341 Y scie scopus kci ART002877520 000831026100002 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article; Early Access FLUORESCENCE ASSOCIATION PLATFORM MODEL One-step homogeneous immunoassay Switching peptide Influenza-A and influenza-B virus Lateral flow immunoassay