이정일 박지헌 김영림 권기학 황혜원 정가영 이주엽 선정윤 박종웅 신재호 옥명렬 2024-01-19T11:31:04Z 2024-01-19T11:31:04Z 2022-02-17 2022-09 1673-5374 https://pubs.kist.re.kr/handle/201004/114770 Nitric oxide (NO) has been shown to promote revascularization and nerve regeneration after peripheral nerve injury. However, in vivo application of NO remains challenging due to the lack of stable carrier materials capable of storing large amounts of NO molecules and releasing them on a clinically meaningful time scale. Recently, a silica nanoparticle system capable of reversible NO storage and release at a controlled and sustained rate was introduced. In this study, NO-releasing silica nanoparticles (NO-SNs) were delivered to the peripheral nerves in rats after acute crush injury, mixed with natural hydrogel, to ensure the effective application of NO to the lesion. Microangiography using a polymer dye and immunohistochemical staining for the detection of CD34 (a marker for revascularization) results showed that NO-releasing silica nanoparticles increased revascularization at the crush site of the sciatic nerve. The sciatic functional index revealed that there was a significant improvement in sciatic nerve function in NO-treated animals. Histological and anatomical analyses showed that the number of myelinated axons in the crushed sciatic nerve and wet muscle weight excised from NO-treated rats were increased. Moreover, muscle function recovery was improved in rats treated with NO-SNs. Taken together, our results suggest that NO delivered to the injured sciatic nerve triggers enhanced revascularization at the lesion in the early phase after crushing injury, thereby promoting axonal regeneration and improving functional recovery. ？ 2022 Neural Regeneration Research. All rights reserved. English Neural Regeneration Research Delivery of nitric oxide-releasing silica nanoparticles for in vivo revascularization and functional recovery after acute peripheral nerve crush injury Article 10.4103/1673-5374.335160 1 Neural Regeneration Research, v.17, no.9, pp.2043 - 2049 Neural Regeneration Research 17 9 2043 2049 Y scie scopus 000802184600037 2-s2.0-85124870434 Cell Biology Neurosciences Cell Biology Neurosciences & Neurology Article MOTOR FUNCTION REGENERATION SYNTHASE CELL VASCULARIZATION INHIBITION EXPRESSION EFFICACY REPAIR BONE nitric oxide neural regeneration revascularization nerve injury silica nanoparticle