Takahashi, Kanako Shima, Takeru Soya, Mariko Yook, Jang Soo Koizumi, Hikaru Jesmin, Subrina Saito, Tsuyoshi Okamoto, Masahiro Soya, Hideaki 2024-01-19T11:31:11Z 2024-01-19T11:31:11Z 2022-11-04 2022-08 0028-3835 https://pubs.kist.re.kr/handle/201004/114775 Introduction: Exercise becomes a stress when performed at an intensity above the lactate threshold (LT) because at that point the plasma adrenocorticotropic hormone (ACTH), a marker of stress response, increases. It is possible that the exercise-induced ACTH response is regulated at least by arginine vasopressin (AVP) and possibly by corticotropin-releasing hormone (CRH), but this remains unclear. To clarify the involvement of these factors, it is useful to intervene pharmacologically in the regulatory mechanisms, with a physiologically acceptable exercise model. Methods: We used a special stress model of treadmill running (aerobic exercise) for male Wistar rats, which mimic the human physiological response, where plasma ACTH levels increase at just above the LT for 30 min. Animals were administered the AVP V-1b receptor antagonist SSR149415 (SSR) and/or the CRH type 1 receptor antagonist CP154526 (CP) intraperitoneally before the exercise, which allowed the monitoring of exercise-induced ACTH response. Immunohistochemical evaluation of activated AVP and CRH neurons with exercise was performed for the animals' hypothalami. Results: A single injection of either antagonist, SSR or CP, resulted in inhibited ACTH levels after exercise stress. Moreover, the combined injection of SSR and CP strongly suppressed ACTH secretion during treadmill running to a greater extent than each alone. The running-exercise-induced activation of both AVP and CRH neurons in the hypothalamus was also confirmed. Conclusion: These results lead us to hypothesize that AVP and CRH are cooperatively involved in exercise-induced ACTH response just above the LT. This may also reflect the stress response with moderate-intensity exercise in humans. (C) 2021 S. Karger AG, Basel English S. Karger AG Exercise-Induced Adrenocorticotropic Hormone Response Is Cooperatively Regulated by Hypothalamic Arginine Vasopressin and Corticotrophin-Releasing Hormone Article 10.1159/000521237 1 Neuroendocrinology, v.112, no.9, pp.894 - 903 Neuroendocrinology 112 9 894 903 N scie scopus 000868579800006 2-s2.0-85123534962 Endocrinology & Metabolism Neurosciences Endocrinology & Metabolism Neurosciences & Neurology Article PITUITARY-ADRENAL AXIS NONPEPTIDE ANTAGONIST ANAEROBIC THRESHOLD V-1B RECEPTORS ACTH-SECRETION RUNNING STRESS ACTIVATION CP-154,526 SSR149415 ENDURANCE Arginine vasopressin Corticotrophin-releasing hormone Hypothalamus Running exercise Stress