최만호 2024-01-19T11:31:14Z 2024-01-19T11:31:14Z 2022-10-18 2022-08 2093-596X https://pubs.kist.re.kr/handle/201004/114778 Accurate measurement of cortisol is critical in adrenal insufficiency as it reduces the risk associated with misdiagnosis and supportsthe optimization of stress dose. Comprehensive assays have been developed to determine the levels of bioactive free cortisol andtheir clinical and analytical efficacies have been extensively discussed because the level of total cortisol is affected by changes in thestructure or circulating levels of corticoid-binding globulin and albumin, which are the main reservoirs of cortisol in the human body. Antibody-based immunoassays are routinely used in clinical laboratories; however, the lack of molecular specificity in cortisol assessment limits their applicability to characterize adrenocortical function. Improved specificity and sensitivity can be achieved bymass spectrometry coupled with chromatographic separation methods, which is a cutting-edge technology to measure individual aswell as a panel of steroids in a single analytical run. The purpose of this review is to introduce recent advances in free cortisol measurement from the perspectives of clinical specimens and issues associated with prospective analytical technologies. English 대한내분비학회 Clinical and Technical Aspects in Free Cortisol Measurement Article 10.3803/EnM.2022.1549 1 Endocrinology and Metabolism, v.37, no.4, pp.599 - 607 Endocrinology and Metabolism 37 4 599 607 Y scie scopus kci ART002868350 000875108100004 Endocrinology & Metabolism Endocrinology & Metabolism Review 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE-1 CHROMATOGRAPHY-MASS SPECTROMETRY CORTICOSTEROID-BINDING GLOBULIN NIGHTTIME SALIVARY CORTISOL URINARY FREE CORTISOL SERUM CORTISOL IMMUNOASSAY INTERFERENCE CUSHINGS-SYNDROME PLASMA-CORTISOL DIAGNOSIS Cortisol Mass spectrometry Immunoassay Saliva Adrenal insufficiency