Song, Zhiquan Park, Jun-Hee Kim, Hong-Rae Lee, Ga-Yeon Kang, Min-Jung Kim, Moo-Hwan Pyun, Jae-Chul 2024-01-19T11:32:08Z 2024-01-19T11:32:08Z 2022-08-04 2022-08 0003-2654 https://pubs.kist.re.kr/handle/201004/114821 In this study, parylene-C films from plasma deposition as well as thermal deposition were pyrolyzed to prepare a carbon electrode for application in electrochemical immunoassays. Plasma deposition could prepare parylene-C in a faster deposition rate and more precise control over the thickness in comparison with the conventional thermal deposition. To analyze the influence of the deposition method, the crystal and electronic structures of the pyrolyzed parylene-C films obtained via both deposition methods were compared using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. For application as a carbon electrode in immunoassays, the electrochemical properties of the pyrolyzed carbon films from two both deposition methods were analyzed, including the double layer capacitance (2.10 mu F cm(-2) for plasma deposition and 2.20 mu F cm(-2) for thermal deposition), the apparent electron transfer rate (approximately 1.1 x 10(-3) cm s(-1) for both methods), and the electrochemical window (approximately -1.0 similar to 2.1 V for both methods). Finally, the applicability of the pyrolyzed carbon electrode from parylene-C was demonstrated for the diagnosis of human hepatitis-C using various amperometric methods, such as cyclic voltammetry, chronoamperometry, square-wave voltammetry and differential pulse voltammetry. English Royal Society of Chemistry Carbon electrode obtained via pyrolysis of plasma-deposited parylene-C for electrochemical immunoassays Article 10.1039/d2an00854h 1 Analyst, v.147, no.16, pp.3783 - 3794 Analyst 147 16 3783 3794 N scie scopus 000829458100001 Chemistry, Analytical Chemistry Article AMORPHOUS-CARBON CYCLIC VOLTAMMETRY TRANSFER KINETICS GRAPHITE SPECTROSCOPY RAMAN TEMPERATURE FILMS FTIR POLYMERIZATION