Ng, Gavin Yong-Quan Sheng, Dominic Paul Lee Kok Bae, Han-Gyu Kang, Sung Wook Fann, David Yang-Wei Park, Jinsu Kim, Joonki Alli-Shaik, Asfa Lee, Jeongmi Kim, Eunae Park, Sunyoung Han, Jeung-Whan Karamyan, Vardan Okun, Eitan Dheen, Thameem Hande, Manoor Prakash Vemuganti, Raghu Mallilankaraman, Karthik Lim, Lina H. K. Kennedy, Brian K. Drummond, Grant R. Sobey, Christopher G. Gunaratne, Jayantha Mattson, Mark P. Foo, Roger Sik-Yin Jo, Dong-Gyu Arumugam, Thiruma V. 2024-01-19T11:32:50Z 2024-01-19T11:32:50Z 2022-04-21 2022-08 2509-2715 https://pubs.kist.re.kr/handle/201004/114854 Intermittent fasting (IF) remains the most effective intervention to achieve robust anti-aging effects and attenuation of age-related diseases in various species. Epigenetic modifications mediate the biological effects of several environmental factors on gene expression; however, no information is available on the effects of IF on the epigenome. Here, we first found that IF for 3 months caused modulation of H3K9 trimethylation (H3K9me(3)) in the cerebellum, which in turn orchestrated a plethora of transcriptomic changes involved in robust metabolic switching processes commonly observed during IF. Second, a portion of both the epigenomic and transcriptomic modulations induced by IF was remarkably preserved for at least 3 months post-IF refeeding, indicating that memory of IF-induced epigenetic changes was maintained. Notably, though, we found that termination of IF resulted in a loss of H3K9me(3) regulation of the transcriptome. Collectively, our study characterizes the novel effects of IF on the epigenetic-transcriptomic axis, which controls myriad metabolic processes. The comprehensive analyses undertaken in this study reveal a molecular framework for understanding how IF impacts the metabolo-epigenetic axis of the brain and will serve as a valuable resource for future research. English SPRINGER Integrative epigenomic and transcriptomic analyses reveal metabolic switching by intermittent fasting in brain Article 10.1007/s11357-022-00537-z 1 Geroscience, v.44, pp.2171 - 2194 Geroscience 44 2171 2194 N scie scopus 000777219700001 Geriatrics & Gerontology Geriatrics & Gerontology Article; Early Access WEB SERVER EXPRESSION METHYLATION HOMEOSTASIS GENERATION OXIDATION H3K9ME3 MUSCLE MEMORY Cerebellum Epigenetics Intermittent fasting Metabolism Transcriptomics