Moon, Keumok Hwang, Sungmin Lee, Hyeon-Jeong Jo, Eunhye Kim, Jeong Nam Cha, Jaeho 2024-01-19T11:33:08Z 2024-01-19T11:33:08Z 2022-08-25 2022-07 1664-302X https://pubs.kist.re.kr/handle/201004/114867 Effective antibacterial substances of Aralia continentalis have anti-biofilm and bactericidal activity to the oral pathogen Streptococcus mutans. In this study, three compounds extracted from A. continentalis were identified as acanthoic acid, continentalic acid, and kaurenoic acid by NMR and were further investigated how these diterpenoids affect the physiology of the S. mutans. When S. mutans was exposed to individual or mixed fraction of diterpenoids, severe growth defects and unique morphology were observed. The proportion of unsaturated fatty acids in the cell membrane was increased compared to that of saturated fatty acids in the presence of diterpenoids. Genome-wide gene expression profiles with RNA-seq were compared to reveal the mode of action of diterpenoids. Streptococcus mutans commonly enhanced the expression of 176 genes in the presence of the individual diterpenoids, whereas the expression of 232 genes was considerably reduced. The diterpenoid treatment modulated the expression of genes or operon(s) involved in cell membrane synthesis, cell division, and carbohydrate metabolism of S. mutans. Collectively, these findings provide novel insights into the antibacterial effect of diterpenoids to control S. mutans infection, which causes human dental caries. English Frontiers Media S.A. Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling Article 10.3389/fmicb.2022.945023 1 Frontiers in Microbiology, v.13 Frontiers in Microbiology 13 Y scie scopus 000838307300001 Microbiology Microbiology Article ENVELOPE STRESS-RESPONSE STREPTOCOCCUS-MUTANS ACID BIOSYNTHESIS RESISTANCE VIRULENCE SEQUENCE SYSTEMS ROOTS Aralia continentalis diterpenoids antibacterial activity Streptococcus mutans transcriptome analysis