Baek, Jieun Ryu, Bokyeong Kim, Jin Lee, Seul-Gi Oh, Min-Seok Hong, Ki-Sung Kim, Eun-Young Kim, C-Yoon Chung, Hyung-Min 2024-01-19T11:33:21Z 2024-01-19T11:33:21Z 2022-08-11 2022-07 2227-9059 https://pubs.kist.re.kr/handle/201004/114879 Background: Rotator cuff tears (RCTs) induce chronic muscle weakness and shoulder pain. Treatment of RCT using surgery or drugs causes lipid infiltration and fibrosis, which hampers tissue regeneration and complete recovery. The pluripotent stem cell-derived multipotent mesenchymal stem cells (M-MSCs) represent potential candidate next-generation therapies for RCT. Methods: The difference between M-MSCs and adult-MSCs was compared and analyzed using next-generation sequencing (NGS). In addition, using a rat model of RCT, the muscle recovery ability of M-MSCs and adult-MSCs was evaluated by conducting a histological analysis and monitoring the cytokine expression level. Results: Using NGS, it was confirmed that M-MSC was suitable for transplantation because of its excellent ability to regulate inflammation that promotes tissue repair and reduced apoptosis and rejection during transplantation. In addition, while M-MSCs persisted for up to 8 weeks in vivo, they significantly reduced inflammation and adipogenesis-related cytokine levels in rat muscle. Significant differences were also confirmed in histopathological remission. Conclusions: M-MSCs remain in the body longer to modulate immune responses in RCTs and have a greater potential to improve muscle recovery by alleviating acute inflammatory responses. This indicates that M-MSCs could be used in potential next-generation RCT therapies. English MDPI AG Immunomodulation of Pluripotent Stem Cell-Derived Mesenchymal Stem Cells in Rotator Cuff Tears Model Article 10.3390/biomedicines10071549 1 Biomedicines, v.10, no.7 Biomedicines 10 7 Y scie scopus 000831929300001 Biochemistry & Molecular Biology Medicine, Research & Experimental Pharmacology & Pharmacy Biochemistry & Molecular Biology Research & Experimental Medicine Pharmacology & Pharmacy Article INFILTRATION GENE rotator cuff tears (RCT) pluripotent stem cell (PSC) mesenchymal stem cell (MSC) inflammation treatment