Ko, Kyungmin Lee, Jin-Hyung Lee, Hyunjoo Jenny Oh, Soo-Jin Chun, Ye Eun Kim, Tae Song Lee, C. Justin Yoon, Eui-Sung Yun, Kwang-Seok Cho, Il-Joo 2024-01-19T11:40:14Z 2024-01-19T11:40:14Z 2022-03-07 2015 1084-6999 https://pubs.kist.re.kr/handle/201004/115066 In this work, we present a piezoelectric micromachined ultrasonic transducer (pMUT) array for local stimulation on cultured cells with a high spatial resolution for the first time. We used a bulk piezoelectric film that has a higher piezoelectric coefficient (d(31)) than that of the thin film materials to achieve high acoustic power within a small membrane. The 500-mu m-wide and 55-mu m-thick transducer membrane exhibits a resonant frequency of 780 kHz, which is within the effective frequency range for stimulating cells. We also demonstrate successful in vitro experiments by stimulating cells using the fabricated pMUT array and verifying the stimulation using fluorescence calcium imaging. Using fluorescence imaging, we observed an increase in Ca2+ level of TRPA1 expressing HEK293T cells under ultrasound sonication, which confirms that TRPA1 channel in HEK293T cells was activated by ultrasound. English IEEE MICROMACHINED ULTRASOUND TRANSDUCER ARRAY FOR CELL STIMULATION WITH HIGH SPATIAL RESOLUTION Conference 1 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp.651 - 654 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS) 651 654 US Estoril, PORTUGAL 2015-01-18 2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015) 000370382900172 2-s2.0-84931057991 Proceedings Paper