Park, Chang Gyun Jun, Indong Lee, Sangmin Ryu, Chang Seon Lee, Sang-Ah Park, Jaeho Han, Hyung-Seop Park, Honghyun Manz, Andreas Shin, Heungsoo Kim, Young Jun 2024-01-19T12:01:33Z 2024-01-19T12:01:33Z 2022-05-24 2022-06 1613-6810 https://pubs.kist.re.kr/handle/201004/115162 Numerous methods have been introduced to produce 3D cell cultures that can reduce the need for animal experimentation. This study presents a unique 3D culture platform that features bioinspired strands of electrospun nanofibers (BSeNs) and aquatic cell lines to compensate for shortcomings in the current cell spheroid generation techniques. The use of BSeNs in 3D zebrafish liver cell cultures is found to improve liver and reproductive functions through spheroid-based in vitro assays such as whole transcriptome sequencing and reproductive toxicity testing, with optimized properties exhibiting results similar to those obtained for fish embryo acute toxicity (FET, OECD TG 236) following exposure to environmental endocrine-disrupting chemicals (17 beta-Estradiol (E2), 4-hydroxytamoxifen (4-HT), and bisphenol compounds (bisphenol A (BPA) and 9,9-Bis(4-hydroxyphenyl)fluorene (BPFL)). These findings indicate that the beneficial effects of bioinspired materials that closely mimic ECM environments can yield efficient zebrafish cells with intrinsic functions and xenobiotic metabolism similar to those of zebrafish embryos. As a closer analog for the in vivo conditions that are associated with exposure to potentially hazardous chemicals, the straightforward culture model introduced in this study shows promise as an alternative tool that can be used to further eco-environmental assessment. English Wiley - V C H Verlag GmbbH & Co. Integration of Bioinspired Fibrous Strands with 3D Spheroids for Environmental Hazard Monitoring Article 10.1002/smll.202200757 1 Small, v.18, no.22 Small 18 22 N scie scopus 000791260300001 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article IN-VITRO ZEBRAFISH EMBRYOS DRUG DISCOVERY TOXICITY 21ST-CENTURY TESTS MODEL fibrous strands spheroids biomimetics environmental hazard assessment