Anjong, Tikum Florence Choi, Honghwan Yoo, Jounghyun Bak, Yecheol Cho, Yuri Kim, Dojin Lee, Seokyung Lee, Kangwon Kim, Bong-Gi Kim, Sehoon 2024-01-19T12:01:48Z 2024-01-19T12:01:48Z 2022-04-03 2022-06 1613-6810 https://pubs.kist.re.kr/handle/201004/115175 Afterglow is superior to other optical modalities for biomedical applications in that it can exclude the autofluorescence background. Nevertheless, afterglow has rarely been applied to the high-contrast "off-to-on" activatable sensing scheme because the complicated afterglow systems hamper the additional inclusion of sensory functions while preserving the afterglow luminescence. Herein, a simple formulation of a multifunctional components-incorporated afterglow nanosensor (MANS) is developed for the superoxide-responsive activatable afterglow imaging of cisplatin-induced kidney injury. A multifunctional iridium complex (Ir-OTf) is designed to recover its photoactivities (phosphorescence and the ability of singlet oxygen-generating afterglow initiator) upon exposure to superoxide. To construct the nanoscopic afterglow detection system (MANS), Ir-OTf is incorporated with another multifunctional molecule (rubrene) in the polymeric micellar nanoparticle, where rubrene also plays dual roles as an afterglow substrate and a luminophore. The multiple functions covered by Ir-OTf and rubrene renders the composition of MANS quite simple, which exhibits superoxide-responsive "off-to-on" activatable afterglow luminescence for periods longer than 11 min after the termination of pre-excitation. Finally, MANS is successfully applied to the molecular imaging of cisplatin-induced kidney injury with activatable afterglow signals responsive to pathologically overproduced superoxide in a mouse model without autofluorescence background. English WILEY-V C H VERLAG GMBH Multifunction-Harnessed Afterglow Nanosensor for Molecular Imaging of Acute Kidney Injury In Vivo Article 10.1002/smll.202200245 1 SMALL, v.18, no.22 SMALL 18 22 N scie scopus 000771352300001 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article IRIDIUM(III) COMPLEXES FLUORESCENCE PROBE RATIONAL DESIGN NITRIC-OXIDE L-CARNITINE SUPEROXIDE CISPLATIN CELLS afterglow multifunctional iridium complexes phosphorescence superoxide-activatable afterglow systems