Kim, Hakchun An, Hyun Ji Park, Junhee Lee, Yohan Kim, Min Seob Lee, Seungki Kim, Nam Dong Song, Jihwan Choi, Inhee 2024-01-19T12:02:22Z 2024-01-19T12:02:22Z 2022-06-09 2022-05 2196-5404 https://pubs.kist.re.kr/handle/201004/115201 Reactive oxygen species (ROS) regulate various physiological and pathological conditions in cells by interacting with signaling molecules and inducing oxidative stress. Therefore, sensitive monitoring of ROS levels in living cells is important to track cellular state and study the complex role of ROS in the development of various pathologies. Herein, we present an optically tunable plasmonic interface covered with graphene to monitor cellular ROS levels with superior sensitivity and cellular comfortability. As a sensing principle, we employed plasmon resonance energy transfer (PRET)-based spectral quenching dips modulated by redox-active cytochrome c for real-time monitoring. By transferring graphene layers to plasmonic nanoparticles immobilized on a glass substrate, the scattering profiles of the nanoprobes were adjusted in terms of the position, width, and intensity of the peaks to determine the optimal conditions for measuring the PRET signal. Using the optimized graphene-covered plasmonic nanoprobe, we obtained calibration curves over a wide concentration range from femtomoles to millimoles for hydrogen peroxide based on the change in the PRET signal. Before monitoring cellular ROS, we confirmed that a high density of cells adhered well to the graphene-covered plasmonic interface by observing immunofluorescence images of the cytoskeleton of the immobilized cells. Finally, we monitored the real-time ROS generated by the cells under oxidative stress conditions by directly measuring the spectral changes of the probes around the cells. We believe that the proposed graphene-covered tunable plasmonic interface has versatile applicability for investigating cellular stress and disease progression by monitoring ROS levels under various cellular conditions. English Springer | Korea Nano Technology Research Society Ultrasensitive and real-time optical detection of cellular oxidative stress using graphene-covered tunable plasmonic interfaces Article 10.1186/s40580-022-00315-9 1 Nano Convergence, v.9, no.1 Nano Convergence 9 1 Y scie scopus kci 000800948000001 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Science & Technology - Other Topics Materials Science Physics Article OXYGEN SPECIES ROS HYDROGEN-PEROXIDE CYTOCHROME-C RESONANCE ENERGY NANOPARTICLES LUCIGENIN CELLS FLUORESCENCE PROMOTE Graphene Oxidative stress Plasmonic nanoparticles Plasmonic resonance energy transfer Reactive oxygen species