Cai, Wen Ting Kim, Wha Young Kwak, Myung Ji Rim, Haeun Lee, Seung Eun Riecken, Lars Bjorn Morrison, Helen Kim, Jeong-Hoon 2024-01-19T12:03:26Z 2024-01-19T12:03:26Z 2022-04-05 2022-05 0022-3042 https://pubs.kist.re.kr/handle/201004/115257 Repeated injections of psychomotor stimulants like amphetamine (AMPH) to rodents can induce behavioral sensitization, which represents a long-lasting craving that is usually observed in human addicts. Behavioral sensitization is characteristically maintained for a long duration, accompanied by structural plasticity in some brain areas involved in reward circuitry. For example, it increased dendritic spine densities in the nucleus accumbens (NAcc), which is considered to reflect neurophysiological changes at this site, leading to addictive behaviors. The ezrin, radixin, and moesin (ERM) proteins regulate spine maturity by modifying their phosphorylation at the C-terminal region. We previously showed that ERM phosphorylation is reduced by AMPH in the NAcc core, suggesting that ERM-mediated spine changes at this site might be associated with AMPH sensitization. To test this hypothesis, we administered AMPH to rats according to a sensitization development schedule, with lentivirus encoding a phosphomimetic pseudo-active mutant of radixin (Rdx T564D) in the NAcc core, and examined dendritic spines at this site. We found that compared to acute AMPH, AMPH sensitization increased thin spine density with a similar ratio of filopodia-like to mature thin spines. However, with Rdx T564D, the density of thin spines increased, with augmented filopodia-like thin spines, resulting in no AMPH sensitization. These results indicate that Rdx T564D forces thin spines to immaturity and thereby inhibits AMPH sensitization, for which an increase in mature thin spines is normally necessary. These findings provide significant clues to our understanding of the role of dendritic spines in mediating the development of psychomotor stimulant addiction. English WILEY Disruption of amphetamine sensitization by alteration of dendritic thin spines in the nucleus accumbens core Article 10.1111/jnc.15582 1 JOURNAL OF NEUROCHEMISTRY, v.161, no.3, pp.266 - 280 JOURNAL OF NEUROCHEMISTRY 161 3 266 280 N scie scopus 000761737200001 2-s2.0-85124557527 Biochemistry & Molecular Biology Neurosciences Biochemistry & Molecular Biology Neurosciences & Neurology Article AMPA RECEPTOR STRUCTURAL PLASTICITY ERM PROTEINS SYNAPTIC PLASTICITY EXPERIENCE EZRIN/RADIXIN/MOESIN PHOSPHORYLATION TELENCEPHALIN EXPRESSION INCREASES amphetamine locomotor sensitization nucleus accumbens radixin dendritic spine